The Brazil international sustained the injury in the 21st-minute of the quarter-final, first-leg defeat at the Parc des Princes and missed the weekend Ligue 1 clash against Nice.
He was included in the initial squad for the trip to Spain but will instead remain in Paris to work on his rehabilitation.
A PSG statement read: "Having initially being named in the squad for the quarter-final against Barcelona, Brazilian defender Thiago Silva will not ultimately make the trip.
"The captain of Paris Saint-Germain will therefore remain at the Ooredoo training centre to work on his return to action."
PSG are already without the injured Thiago Motta and suspended Serge Aurier as they attempt to overcome a 3-1 deficit from the first game.
They will, however, welcome back Zlatan Ibrahimovic and Marco Verratti, who both sat out the first leg as they served one-match bans.
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