The Spain international missed Saturday's 3-0 win over Eibar and is believed to have been left out of the Group F encounter merely as a precaution with Real Madrid on the horizon in La Liga this weekend.
"Sergio Busquets has a contusion on the left hip and will be left out from the party today as a precaution," read a statement on the club's official Twitter account.
His absence could see Barca coach Luis Enrique name a midfield three of Xavi, Javier Mascherano and Ivan Rakitic at Camp Nou.
The Catalans will be keen to bounce back after their 3-2 defeat to Paris Saint-Germain last time out, although they can take solace from their recent home record in this competition.
The Catalan giants have lost just one of their previous 29 Champions League matches at Camp Nou - a 3-0 reverse at the hands of Bayern Munich in the semi-final second leg in 2012-13.
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